FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2227-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2227-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7651447
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTG725240.9478468582478581TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG316790.4140262619606461TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATG231060.30198209567419076TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGG109270.14280958882679315TruSeq Adapter, Index 8 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT77910.10182387723524713TruSeq Adapter, Index 8 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA88800.055.45407560
TGGCGGG120450.050.44233749
TCGGGGA39300.049.7819142
CGGGGAT38700.049.5589343
TGCCGGG34100.047.72554449
GCCGGCG15800.047.40334750
ATGCCGG25700.046.5741948
TGGGGGT96600.046.4838257
GGTGGAA107050.045.93481461
CGGGGTG115550.045.251552
GCCGGGG35800.045.16596250
CCGGGGT30950.044.7802851
AGTCGGA23200.044.236251
TGCCGGC22100.044.18391849
GGTGGCG88250.043.34687447
GTGGCGG88600.043.17563248
GTGGAAA137050.043.05571762
GGCGGGG151800.042.6763250
GGGTGGC92700.042.549746
CCGGCGT18300.042.45747851
Produced by FastQC (version 0.11.5)