FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2226-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2226-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7973892
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTG546840.6857880693643706TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTG269660.33817864601125774TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGATG168420.2112142978610696TruSeq Adapter, Index 7 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA98670.12374132982989987TruSeq Adapter, Index 7 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGATG92440.115928332111847TruSeq Adapter, Index 7 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG86410.10836615294012009TruSeq Adapter, Index 7 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTT82090.10294847233947989TruSeq Adapter, Index 7 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA92850.053.75124760
ATGCCGG33100.048.42709448
TGGCGGG135750.046.2523249
CGGGGAT28600.045.27793543
TCGGGGA29300.045.15180642
GGTGGAA111450.045.06327461
GCGTGGA32700.044.5242460
GTGGAAA147400.044.1163162
CGGGGTG140000.042.2733352
TGCCGGC26050.041.91773249
AAAAAGG221300.041.7270
TGGGGGT116950.041.71703757
GCCGGCG19600.041.24837550
CGGGGGT110100.041.1972943
GGTGGCG86450.041.17246647
GGCGGGG156950.041.0305650
AGTCGGA20250.040.9957961
TCGCGGA18050.040.71861642
ATCGGGG208650.040.67656341
CATCGCG66000.040.51355740
Produced by FastQC (version 0.11.5)