FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2225-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2225-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6965174
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1259801.8087128907332395TruSeq Adapter, Index 6 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTGTG168160.24142971876940905TruSeq Adapter, Index 6 (97% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA113270.16262336016300527TruSeq Adapter, Index 6 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATG109160.15672257433913353TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTG81400.1168671450275327TruSeq Adapter, Index 6 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT76580.1099470020418729TruSeq Adapter, Index 6 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA61850.051.60702560
GGGTGGA66000.050.8013560
GGGCGTG57900.047.3904958
GTGGGCG53100.046.66512756
GTGGAAA145350.045.72604462
TGGGCGT62100.045.5379557
AGTCGGA23050.044.980771
TGGGGGT76650.044.382157
GTGGGGG80650.044.17709456
GGCGTGA47850.043.373759
AAAAAGG233450.042.9820970
TCGGCGT48850.042.91567257
GGGGGTG78350.042.83840658
GCCGTGG41850.042.6509250
GTCGGCG36450.042.63233656
GCCGGGG42600.042.3108150
GCGTGAA81450.042.19627460
CGTGGGC31100.041.9761155
GGTGGAA81350.041.90396561
GGAAAAA189400.041.44794564
Produced by FastQC (version 0.11.5)