FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2223-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2223-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9220147
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG194920.21140660772545167TruSeq Adapter, Index 10 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGG50150.042.1518849
GGGTGGA42500.040.6807560
GGCGGGG58050.037.80212850
GCCGGGG17600.036.58946650
CGGGGTG57000.035.4898352
TGGGGGT47150.035.03573657
TCGGGGA18850.034.90578542
TGCCGGG18750.034.9052949
ATCGGGG93700.034.69980641
GCGGGGT42500.034.66922451
CGGGGGT49950.034.3329843
GTGGGGG61250.033.88437756
TATCGGG112100.032.6258340
CGGGGAT19550.032.58183743
GGGTGGC40950.032.47735246
ATGCCGG18000.031.10988648
GGGTGGG69500.030.41606754
TCGGGGG60350.030.38819542
GTGGCGG43400.029.51496548
CACTAGC164500.029.31799532
Produced by FastQC (version 0.11.5)