FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2222-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2222-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056132
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGGTG325090.46071983914133124TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG214160.30350906133842165TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATG210230.2979394376409058TruSeq Adapter, Index 5 (97% over 41bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG117450.1664509677540046TruSeq Adapter, Index 5 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGGTG101320.14359141807437842TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG99520.1410404453884933TruSeq Adapter, Index 5 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGATG91390.1295185520905788TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGTTG74900.1061488078737756TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGTTG70860.10042329140101121TruSeq Adapter, Index 5 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA25450.048.438331
GCGTGGA53050.047.63278660
GGGTGGA123400.045.2092560
ATGCCGG71200.044.63340448
GTGGAAA193650.042.18300262
AAAAAGG227050.041.21867870
GGTGGAA137300.040.9382461
GATGCCG40900.040.0476247
GGATGCC44700.039.14863646
GTCGGAA28600.038.43762
GGGCGTG54400.038.2156958
TGGGCGT56800.036.78579357
CGTGGAA69200.036.667961
GTATGCC79700.036.53584346
TATGCCG77550.036.4656347
CCGGGGT67300.036.40299651
TGCCGGG68850.036.396849
CGGATGC37000.036.0393945
GGCGGAT42600.035.73829343
GCGGATG47350.035.1836744
Produced by FastQC (version 0.11.5)