FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2221-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2221-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6752037
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3511125.200089987658539TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTGTG434160.6430059550917746TruSeq Adapter, Index 4 (97% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC219430.3249834087105862TruSeq Adapter, Index 4 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA175990.26064726837249264TruSeq Adapter, Index 4 (100% over 39bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC128870.19086092093393445TruSeq Adapter, Index 4 (100% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTTTG120860.17899783428319485TruSeq Adapter, Index 4 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCGCGTATG113290.1677864028292499TruSeq Adapter, Index 4 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC107560.15930007492553727TruSeq Adapter, Index 4 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATG71950.10656043502131282TruSeq Adapter, Index 4 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA43600.058.7251631
GGGTGGA94400.055.76068560
GCGTGGA112300.053.9784560
GTGGAAA213050.053.66855262
CGTGGAA119500.051.0776561
GGTGGAA105500.050.39153761
AAAAAGG454000.049.6072370
GGGCGTG92950.049.06223358
GTCGGAA49050.048.894662
CGTCGGC78200.048.2910955
GTCGGCG74850.048.25476556
TCGTCGG74250.048.22047454
TCGGCGT106750.048.19497357
TGGGGGT106150.048.00579557
TGGAAAA271550.047.9710163
GGAAAAA289650.047.96994864
GGGGGTG104600.047.9141358
GCCGTGG77600.047.67223750
CGTGAAA168950.047.64550861
GGCGTGA95000.047.5982759
Produced by FastQC (version 0.11.5)