FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2220-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2220-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6547531
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGGTG157940.24122069830597215TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGGGGGGTG102810.15702102059539694TruSeq Adapter, Index 3 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGTTG83940.128200996681039TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG75650.11553973551251609TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGGGGATG70790.10811709024363535TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGGATG70120.10709380375595015TruSeq Adapter, Index 3 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGA64550.051.02086360
GCGTGGA26200.044.48333460
ATGCCGG26400.044.41148448
GGTGGAA78650.042.8975761
CGGGGTG91750.042.5708752
TGGCGGG73950.041.9797549
ATCGGGG76100.041.34561541
GTGGAAA110950.041.2289962
TCGCGGA16250.040.70641342
GGCGGGG78050.040.49250
CCGGGGT30750.040.29141651
CGGGGGT37900.040.17024243
GCGGGGT72050.039.73504651
CGGGGAT17900.039.69149443
CATCGGG93400.039.57048840
ATCGCGG30300.039.1571941
CCGGCGT16800.038.74878351
GCCGGCG14500.038.61947650
TCGGGGG40150.038.529342
CATCGCG54050.038.46317740
Produced by FastQC (version 0.11.5)