FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2219-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2219-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6396295
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTG242890.37973545622895755TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATG109160.17066129689140352TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTG93850.14672556534681405TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG75010.1172710139229038TruSeq Adapter, Index 2 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG35450.042.6501648
GGGTGGA71800.040.11701260
GCGTGGA30100.039.5335360
CGGGGAT22550.038.4909243
TCGGGGA23050.038.2633342
AGTCGGA14800.037.8649561
TCGCGTG12300.036.990648
GGGCGTG29750.036.8222758
TGCCGGG37000.036.2284949
CGCGGAT15900.036.0993943
GCCGGGG36200.035.9656250
CGCGTGT12600.035.55433749
CCGGGGT34850.035.24986651
TCGCGGA16500.035.21091542
TGGCGGG80100.034.4307849
CGGGGTG99450.034.38293552
GATGCCG24500.033.99883747
GGATGCC26750.033.7558546
AAAAAGG149450.033.5819970
GTGGAAA123250.033.39439462
Produced by FastQC (version 0.11.5)