FastQCFastQC Report
Tue 24 Nov 2020
HHGHNAFX2_n01_DS2218-1.dk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHGHNAFX2_n01_DS2218-1.dk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771455
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG924022.450035861491122TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA119130.3158727864975189TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA48340.12817334423982257TruSeq Adapter, Index 1 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA13250.048.637841
GGCGTGA21900.045.8662659
GCGTGGA19050.045.0118360
CGGCGTG27100.044.1684758
CGTCGGC20100.044.0534655
GCGTGAA40450.044.04075660
TCGGCGT27500.043.6532957
GTCGGCG17800.042.4706956
AAAAAGG120100.041.23528370
GCCGTCG48400.040.56701350
TCGTCGG22200.040.35919654
GTCGGAA15250.039.7148322
CGTGAAA45450.039.5808261
GGGCGTG14700.039.5226758
CCGTCGT49900.039.48784351
GGCGTGG19750.038.1001759
TGCCGTG21900.037.0765649
GTGGGCG12850.036.7693756
GCCGTGG16350.036.17632750
GTCGGGG4850.036.08143256
Produced by FastQC (version 0.11.5)