FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28633442
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17338826.055443840806844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1162770.4060880979660077No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG615830.21507368901021398No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA571680.1996546555597472No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC570930.1993927240741787No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG323670.11303915191194966No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT300560.10496816973663173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT343550.069.41637
TAGTAGG343450.069.193736
TAGGTTG343900.069.13019
TGTATAG437600.069.1244914
GTTGTAT356350.069.1111612
TGAGGTA644200.069.05811
GAGGTAG663700.069.013892
GTAGTAG597950.068.958435
TATAGTT397450.068.8762816
GGTAGTA604800.068.6875154
GTATAGT439350.068.5213415
TTGTATA449750.068.4855513
AAGATGC93400.068.362615
CAGACTG413550.068.29999
TAGTAGT159850.068.274426
GACTGAT414900.068.24026511
TAGTTAA391750.068.164718
AGGTAGT609900.068.012083
ATAGTTA394100.067.89072417
CAGTGGC115250.067.811195
Produced by FastQC (version 0.11.5)