FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19816858
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1914490.9660915973662424No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1528470.771297851556488No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1498900.756376212616551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1132510.5714881743614452No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT849980.4289176417371513No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG788580.39793392070529043No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG524740.26479475202375674No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC521040.2629276548280257No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA477180.24079498374565736No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT402650.20318559077326992No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC397240.20045559190059295No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC392480.19805359658932814No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA361950.1826475216202286No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC331140.16710015280928997No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT290920.14680430167082995No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG244080.12316786041460255No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT229700.1159114123944371No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA225730.11390806756550408No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG215910.10895269068386118No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA209290.10561210056609378No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA205820.1038610661690163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT998600.069.3766868
AGTAGGT1004700.069.301747
CAGCACG648400.069.262454
TAGGTTG1000650.069.2209249
GTTGTAT1035250.069.2088212
AGCTTAT1448800.069.192122
GAGGTAG1995900.069.181562
TAGTAGG1002150.069.1647956
GTTGACA869700.069.156218
GCTTATC1451200.069.093433
TATAGTT1157750.069.09093516
TGAGGTA1964250.069.0755841
GTAGTAG1803700.068.998145
TGTATAG1299850.068.9837814
CGTAAAT651050.068.982269
TGTTGAC934300.068.9598517
AGGTTGT1176250.068.9580610
GGTAGTA1814400.068.941964
AGATGCT297700.068.941856
TAGCAGC717250.068.935211
Produced by FastQC (version 0.11.5)