FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17669952
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG2193311.2412653978912902No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1982151.1217630925086837No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1728340.9781237662671636No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT1006080.5693733633232281No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG967190.5473642486408565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG800370.4529553900316198No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA693540.39249682172311506No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC603460.3415176226851097No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT513890.2908270492189226No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC505440.2860449196466408No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG475110.2688801871108648No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC474900.26876134128717494No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC447100.2530284179606147No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA433430.24529212077089965No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG359090.203220699184695No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT339460.19211144433216343No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT324450.1836167976008084No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG271970.15391666032822274No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT269650.15260369694269685No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA268890.15217358824743837No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC258820.1464746480352635No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA254070.14378646868989797No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG218310.12354872271299888No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA211780.11985318352873851No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA208370.11792335372501293No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA200960.11372979394624276No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA192810.1091174441220893No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT182200.10311290036328338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT865550.069.402828
AGTAGGT870600.069.33627
CAGCACG552500.069.2481464
TAGGTTG867950.069.219169
TGTATAG1125200.069.15433514
GAGGTAG1710150.069.1214752
GTTGTAT903400.069.11426512
TAGTAGG870500.069.0566
GTTGACA656300.069.0355818
GTAGTAG1536950.069.0329065
AGCTTAT1096850.069.002852
TATAGTT1005100.069.0007116
AGATGCT246950.068.986786
GCTTATC1097750.068.966193
AAGATGC247350.068.959685
GGTAGTA1548500.068.913154
TGAGGTA1686000.068.909781
TTGGCGA483700.068.8631717
TGTTGAC711550.068.8562817
ATTGGCG543850.068.8262816
Produced by FastQC (version 0.11.5)