FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17380951
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC2626221.5109760104611076No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG2108971.213380096405542No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1726240.9931792569923246No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG1001820.5763896348364367No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT997850.5741055250659185No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA907540.5221463428554628No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC849860.48896058679412885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG814090.4683805851590054No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC677380.3897255104165474No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC669370.38511701689970823No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT651990.3751175640504366No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC634770.3652101660029995No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA608030.34982550724641015No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT491410.28272906355929545No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT470350.27061235026783054No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG455410.26201673314653495No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG452630.26041728096466066No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA432650.24892193758557862No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC403200.2319781006229176No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA394800.22714522352660682No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG372010.2140331676903065No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG311130.1790063155922826No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA306440.17630795921350909No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT301220.1733046713036588No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA290010.16685508175012978No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC252950.14553288827521577No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT245760.14139617561777834No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG240660.13846192880930394No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA238530.13723644925988226No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT235230.1353378189720459No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG226180.1301309692432825No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT215160.12379069476693191No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG213970.12310603717828787No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT213380.1227665850965232No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA211830.12187480420375156No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT211640.1217654891265731No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA209360.12045370820043162No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC204740.11779562579746067No Hit
TCAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGA197910.11386603644415083No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC191710.11029891287306431No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAA190910.10983863886389186No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC190530.10962000870953495No Hit
TGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAG188830.10864192644004346No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG187880.10809535105415118No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA180820.10403343292320426No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC176690.10165726835085147No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA176680.10165151492573679No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT173930.10006932301920649No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT822450.069.3719258
AGTAGGT825950.069.3295067
CAGCACG558850.069.263924
TAGGTTG824850.069.1403969
AGCTTAT1211400.069.1266562
TAGTAGG827050.069.107176
GTTGACA747100.069.0884118
TTGGCGA493100.069.0845217
AAGATGC221400.069.0485845
GAGGTAG1665150.069.046282
TGTATAG1071400.069.0401114
GTAGTAG1497500.069.035265
GCTTATC1213700.069.0328453
TATAGTT962000.068.9837816
GGTAGTA1508950.068.968134
GTTGTAT857150.068.9033712
ACAGTGG326900.068.8841864
TAGTTTG433200.068.820699
TGTTGAC808200.068.8128617
GCTGGCA221950.068.73958610
Produced by FastQC (version 0.11.5)