FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22030797
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1681570.7632815099698844No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1341200.6087841488440023No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1300600.5903554011232549No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1258100.5710642243219798No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT764460.34699607100006413No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG739890.33584350125871526No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA420850.1910280413368613No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC377390.17130111089489863No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG362500.1645423903638166No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC341830.1551600697877612No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT332080.1507344468745275No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC305780.13879661275985614No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC282160.12807525755877103No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA267480.12141185813658943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT909500.069.4236768
AGTAGGT914900.069.366717
TAGTAGG913450.069.159426
TAGGTTG913300.069.123339
CAGCACG570450.069.121044
GTTGTAT942750.069.07555412
TGAGGTA1778650.069.067951
AGCTTAT1150500.069.059932
GAGGTAG1815050.068.9690552
TATAGTT1064800.068.95808416
GCTTATC1153750.068.930193
AGGTTGT1073300.068.87887610
AGATGCT263150.068.810916
GTAGTAG1636250.068.806395
GGTAGTA1644950.068.7976464
GTTGACA703550.068.7643318
TGTATAG1193900.068.68317414
AAGATGC264050.068.6822055
CGTAAAT575650.068.6814659
TTGTATA1196250.068.5745713
Produced by FastQC (version 0.11.5)