FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19549104
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1722480.8811043206890711No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1366700.6991113249998568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1074160.5494676380053019No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1050360.5372931669911828No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT782680.4003661753500314No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG755120.3862683425286397No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA373280.19094481261136062No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC369740.18913398793110928No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG298390.1526361515085295No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC290640.1486717754430075No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT283990.14527008501259187No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC283850.1451984704772147No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA264320.13520824279209934No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC224560.11486971474498268No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT211350.10811237179975103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT943900.069.42438
AGTAGGT950350.069.39647
TAGGTTG946500.069.2189949
GTTGTAT978950.069.2090712
TAGTAGG948450.069.178546
TATAGTT1103650.069.1021816
TGAGGTA1851900.069.08261
AGCTTAT1228100.069.076072
TGTATAG1232900.069.0180614
GAGGTAG1885800.069.016142
CAGCACG597100.068.973874
GTAGTAG1694150.068.970785
GCTTATC1231100.068.966953
AGGTTGT1118450.068.92821510
GTTGACA755850.068.8389818
GGTAGTA1707650.068.835234
AGATGCT268900.068.8193366
TGCTGGC269350.068.796689
CGTAAAT600050.068.7412959
TAGTTTG467950.068.7320869
Produced by FastQC (version 0.11.5)