FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17043429
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1626110.9540979106962573No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1351800.7931502516307019No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1051750.6170999978936164No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG906900.5321112318419022No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG778370.4566980036705055No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT764810.44874185822583007No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA440470.2584397775823163No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC362410.21263913500035705No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC349060.20480620419752385No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT340120.19956078087337942No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG294300.17267651949616478No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC276510.16223847912295114No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC268850.1577440783776551No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA244500.1434570472878433No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227270.13334757929287586No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG218400.12814322751601218No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT205170.12038070507994607No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT190040.11150338350340182No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC189180.11099879020823804No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA175140.10276101129649438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT928500.069.4618
AGTAGGT933100.069.415647
GTTGTAT967200.069.2796712
TAGGTTG931450.069.233499
TAGTAGG931650.069.228286
GAGGTAG1838300.069.1913152
TATAGTT1089850.069.1882616
TGTATAG1212300.069.1564214
TGAGGTA1809550.069.1457061
AGGTTGT1100250.069.0861410
AGCTTAT1085050.069.059132
GGTAGTA1661400.069.0314564
GCTTATC1086600.069.0233463
GTAGTAG1650950.069.0191965
CAGCACG545850.068.9644554
TAGTTTG448250.068.959119
GTTGACA675950.068.9188218
GTATAGT1213100.068.8630615
TGCTGGC257300.068.823799
AGTAGTT449400.068.818747
Produced by FastQC (version 0.11.5)