FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19921982
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1929960.9687590321083515No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1677860.8422153980462387No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1416130.7108379075937323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1152020.5782657568910563No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG976130.4899763487387952No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT960070.48191490184058994No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA542520.2723223020681376No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC476270.23906757871782033No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT447370.2245609899657574No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC444030.22288444995081313No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG386570.19404193819671156No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC351340.17635795474566737No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC339190.17025916397274127No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA302400.15179212590393867No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT269870.13546342929132252No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG269250.1351522152765724No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA232390.11665004014158832No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT228620.11475765814867216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224820.11285021741310679No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC217820.10933651079496007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT1114950.069.434088
AGTAGGT1119850.069.390237
CAGCACG666300.069.2798844
GTTGTAT1149200.069.2630712
TAGGTTG1118100.069.238649
TAGTAGG1118250.069.218236
GAGGTAG2185100.069.15032
TATAGTT1289300.069.1194616
TGAGGTA2151000.069.102461
TGTATAG1438050.069.0991814
AGATGCT298950.069.0617456
GTAGTAG1963650.069.048355
AGCTTAT1293750.069.03492
TTGGCGA590850.069.0321817
AAGATGC299400.069.015845
AGGTTGT1319200.069.0110810
GTTGACA793050.068.978418
GGTAGTA1976800.068.967894
GTATAGT1434500.068.9383815
TAGTTTG531700.068.898659
Produced by FastQC (version 0.11.5)