FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18645501
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1677490.899675476673971No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1552540.8326619917587627No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1404120.753061019921106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1077770.5780322019773027No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG916240.4914000433670299No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT878210.4710037021799522No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA560140.3004156337767486No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC504210.27041912148136965No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT473500.25394866032293795No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC466600.2502480357057716No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC352560.1890858282649525No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC332680.17842373878824708No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA307630.16498886246070835No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG295690.1585851729057857No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT273830.14686116506067604No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG238060.1276769125163223No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA233550.12525809845495706No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT223250.11973397764962175No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC220460.11823763813050665No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG195850.10503874366261332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT1032700.069.435398
AGTAGGT1037750.069.395647
GTTGTAT1078750.069.2126112
TAGGTTG1035250.069.210299
TATAGTT1209350.069.1934616
TAGTAGG1037600.069.193286
GAGGTAG2024550.069.164632
TGTATAG1346850.069.146614
CAGCACG575800.069.129284
TGAGGTA1992300.069.0604251
AGCTTAT1197500.069.0566562
GGTAGTA1827450.069.0217064
GCTTATC1198250.069.009513
GTAGTAG1818200.068.978635
AGGTTGT1225250.068.9654710
AAGATGC280600.068.927055
CGTAAAT578600.068.923929
GTTGACA745700.068.9160318
GTATAGT1346100.068.8974115
CAAGATG280600.068.827134
Produced by FastQC (version 0.11.5)