FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17969347
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG1767050.9833690673345002No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1568470.8728586520144556No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1522260.8471426368470708No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG859990.4785872296861984No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT846520.47109113091310445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG799400.44486869778851734No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA508650.28306537794612124No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC456410.2539936481832089No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC427080.2376714078703027No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT415810.23139961624648908No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC371830.2069246033258749No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA325140.18094146659864713No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC325020.18087468620868638No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG309390.17217654041629893No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG266770.1484583719152399No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT260920.14520282790465341No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC260480.14495796647479733No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT244480.1360539144800309No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA208590.11608101284927047No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG206940.1151627824873102No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA199240.11087770746482886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT940600.069.404937
GTAGGTT935600.069.377088
CAGCACG550450.069.227044
TAGTAGG938700.069.203486
TAGGTTG938050.069.188429
TGTATAG1219600.069.1391714
GTTGTAT978000.069.12363412
GAGGTAG1828800.069.110422
AGCTTAT1145450.069.027132
AGGTTGT1107000.069.0270710
TATAGTT1096800.069.02611516
GTAGTAG1641700.069.015965
TGAGGTA1801250.069.0082551
GGTAGTA1652900.068.9906854
GCTTATC1148750.068.883613
GTTGACA715000.068.8294418
GTATAGT1219950.068.805415
TTGGCGA484100.068.7985817
AGATGCT261400.068.739946
TGCAGGT204400.068.7202415
Produced by FastQC (version 0.11.5)