FastQCFastQC Report
Wed 26 Jun 2019
HHG5LBGXB_n01_AHP01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5LBGXB_n01_AHP01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17782799
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3143711.7678375603300693No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG2278551.2813224734756323No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA1995191.121977479473282No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG1170920.658456523070412No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT1142190.6423004612490981No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA1068640.6009402681771301No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC1004160.5646805095193395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG866170.48708305143639086No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT820190.46122660442824553No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC813610.45752639952799334No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC773780.43512835071689226No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC695070.3908664772064285No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA668060.37567764219794647No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT606610.34112177728601667No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG566680.3186674943578905No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT554740.31195314078509234No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG529160.2975684536500694No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA459090.2581652078505752No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA446240.2509391238128486No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG435960.24515825658266732No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC399740.22479025939617264No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT350930.19734238687621675No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA335150.18846864320965445No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG330840.18604495276587224No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA307900.17314484632031213No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC292510.16449041571014777No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT273220.15364285453600415No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT271020.15240570396145173No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA270490.15210766314121865No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG261240.14690600731639603No Hit
TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGT253160.14236229066076717No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC234680.13197022583452694No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA227320.1278313948214789No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT226170.12718470247569014No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA224170.12606002013518794No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA222740.12525587226172888No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC220890.12421554109676436No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG220370.12392312368823379No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT217540.12233169817642318No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC217490.12230358111791062No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA214690.12072902584120755No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG214470.12060531078375233No Hit
TCAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGA213960.12031851678692426No Hit
CTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGA210390.11831095880912784No Hit
TGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAG208030.11698383364733528No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG205130.1153530442536071No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAA198040.11136604535652683No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA197500.11106238112459126No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC196820.1106799891288205No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA195850.11013451819367694No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA194050.10912230408722497No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT182750.10276784886338759No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA182230.10247543145485702No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA178240.10023169018555514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT838900.069.433038
AGTAGGT843350.069.392037
TAGGTTG839950.069.3174749
CAGCACG513950.069.222544
TAGTAGG842300.069.196696
AGCTTAT1005550.069.07912
GTAGTAG1472800.069.071375
TGTATAG1085250.069.06364414
GGTAGTA1481400.069.0316854
GAGGTAG1640400.069.014312
GCTTATC1007750.068.9845663
GTTGACA624600.068.9845218
AAGATGC249200.068.9677665
TATAGTT974150.068.9477916
TAGCAGC575400.068.876941
GTTGTAT875800.068.828412
TTGGCGA455200.068.8109217
GCTGGCA249900.068.7918710
GCACGTA518050.068.7630546
AGATGCT249950.068.705426
Produced by FastQC (version 0.11.5)