FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27182195
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2789241.0261275809403914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC63450.043.2428322
TTGTACT62800.042.9276161
TCTAGTT87400.031.2412457
GTACTCT92900.029.9352473
ATACGAG107000.028.6706125
CCATTGT161250.025.464684145
CGGGAAC227900.022.6300322
GAACGTA261900.022.2915765
CCGGGAA233200.022.1440541
GGAACGT243150.022.060764
GTATTCA259450.021.9998559
GGGAACG294000.019.1422923
TACTCTA155300.018.6994444
ACGAGAT194500.018.5069667
TATTCAC263050.018.0364199
GGTTAGA163950.017.3577164
TAGTTGT182900.015.7621949
AACGTAT383150.015.6136656
CCCTGAG101050.014.5693475
CTTACAT83300.014.5502111
Produced by FastQC (version 0.11.5)