FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_9361.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_9361.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20525173
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG396270.19306536417500597No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG306870.14950909305368584No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC305970.14907060710280007No Hit
CCCCACCATGCCGTAATGTACCGCGTTTTGATCCTGCTTAGGGCAGGTGG287590.14011574957248837No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA262660.12796968873295247No Hit
GTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCGAACTCT218520.10646438887506576No Hit
GGAAGAGAAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAG206270.10049610787689828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACATT146750.041.9710732
ACATTCA158200.039.1986164
ATGCCGT182900.038.5082178
CTTACAT169650.036.7548261
TTGTACT48500.036.0284041
TGTACTC49350.035.3866232
TTCAGTA186350.033.904657
CATTGTA252750.030.8302145
CATTCAG212800.030.0277375
TACGACT99000.029.8630542
CTCATAT38850.029.8606111
CCGGGAA332650.029.5993371
TACATTC212150.029.266223
CAGTAGC218200.028.494079
CTATTAG85550.027.4426338
GCTCAGA61600.027.4031755
CACGAGT105250.026.7766553
CCATTGT328000.026.654861145
TGGTTCG151150.026.6498933
TCAGTAG242750.025.5215858