FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_9109.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_9109.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21767998
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACGCATAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGACAGGTG540900.2484840360606428No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC519450.23863012115307985No Hit
CTCGGTTTCCCTACGGCTCCGTCTCTTCAACTTAACCTCGCATCATAACG481130.22102629741145696No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG422550.194115232829404No Hit
GGAAGAGAAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAG372350.17105385621590005No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC368400.16923926582499688No Hit
CTGGGTTAGAGTGGCCATAACACAAGGGTAGTATCCCAACAACGTCTCCT300500.1380466867003571No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG264030.12129273440763821No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG259360.11914738323662102No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACT257620.11834804468467885No Hit
GTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACG247220.11357038897192107No Hit
GGAAGACGCAGTGAACTGAAACATCTAAGTAGCTGCAGGAAGAGAAAGCA239160.1098677057945338No Hit
CACGGTTTCAGGTTCTATTTCACTCCCCTCCCGGGGTGCTTTTCACCTTT225460.10357406317292017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT91050.044.276681
CCGGGAA491050.042.584321
CCATTGT383850.039.335297145
CTATTAG75350.037.985388
AACGTAT648200.036.3942766
TTCAGTA111350.035.984767
CGGGAAC607050.035.2269742
GTATTCA698050.034.12149
GGGAACG673850.033.6314283
GGAACGT672800.033.423864
GAACGTA711300.033.378015
ACGTATT724350.033.060297
TACGACT80550.032.566692
CATTGTA302850.032.438663145
CAATTAG66350.032.4361429
ACATTCA129700.031.559444
CCCTACG363000.030.4422579
CTCGGTT360650.030.1166081
TTAGAGT346150.029.7431856
TTATAGC22850.029.5105251
Produced by FastQC (version 0.11.5)