FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_9109.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_9109.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8226689
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT106860.1298942989093182No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC98970.12030356319535113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT68900.043.4672971
GTACTCT71300.042.1716423
TGTACTC74150.040.8535772
TCTAGTT78900.038.9371387
TAGTTGT80650.037.6464849
TACTCTA82400.036.8391154
TACGAAC24900.035.216892
CTCTAGT90900.034.1147236
GAGCATT71700.028.094718
ACTCTAG111200.028.0148745
CACATAA51850.026.8328769
GTACGAA33000.026.8088171
GACCTAC57100.026.776959
AGCATTG75850.026.2719239
TGACCTA60300.024.8743638
AGAGCAT82700.024.7945427
CACTATA47700.024.609999
TTATGTG33150.024.512836145
AACGTAT82800.023.801296
CGACTCA54500.023.2629034
Produced by FastQC (version 0.11.5)