FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_8337.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_8337.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19739353
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA34500.039.8983464
AGTGTTA105400.039.16834145
TCGATCT33600.037.7335743
TCCTGGC146550.030.554867
CCTGGCT156750.028.9380788
CGTGTAT91000.027.82579145
ATCTATT53350.027.0256676
GATCCTG195550.026.8236355
CATTGTA213550.025.345608145
TGATCCT224050.024.2190574
GACATAT44800.023.96885145
CCAAGTG132000.023.934018
GTGATAT93150.023.8977381
TCGTGTT80100.023.822466145
CCATTGT286150.022.845177145
AAAATCG32750.022.7853183
AATACTA49300.022.4835514
AACTTGT42050.021.8813745
CTGGCTC209450.021.588839
CTTTTAC60250.021.5425571
Produced by FastQC (version 0.11.5)