FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_7431.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_7431.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24922654
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCG620450.2489502121242786No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC607910.24391864526145568No Hit
CTAGGTTAGAACCGCGAACAAACCAGGGTGGTATTTCAAGGATGGCTCCA295780.11867917437685409No Hit
CGGAAAGACCCCATGAACCTTTACTGTAGCTTTGCATTGGATTGCGAACC264050.10594778549668105No Hit
CCCGAAACCAGGTGATCTATCCATGGTCAGGATGAAGGTTGGGTAACACC258970.10390947930344818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA23600.050.6498454
TGATCCT186300.040.9080774
GCTCAGA113550.039.8137665
GCTATAT118050.037.984955145
CATTGTA366850.037.91602145
GATCCTG211100.036.9969865
CCGGGAA529650.035.3292771
TCCTGGC218750.034.2169467
CTGGCTC231800.032.074739
GGCTCAG216600.031.038144
TGTCCTA93850.030.3384975
AACGTAT738950.029.7857515
CCTGGCT252750.029.3287458
CAGATTG147250.028.7386678
CAATTAG106550.028.2243359
CCATTGT604600.027.914555145
GTATTCA785300.027.8663678
CGTATTC816900.026.7694427
TATTCAC837000.026.258879
ATCCTGG296650.025.8648056
Produced by FastQC (version 0.11.5)