FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_6751.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_6751.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21781207
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC1483010.6808667673926427No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG1062120.48763137873856116No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT453240.20808764179138464No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC421120.19334098427144097No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT378170.17362215050800445No Hit
CGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGA373290.1713816869744638No Hit
GGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTT349010.1602344626723395No Hit
TGAAGATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTA283400.13011216504209341No Hit
CACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCTGGA265070.1216966534499213No Hit
TGGGAATCTAGCTTATGGAGGGGGATAACTACGGGAAACTGTAGCTAATA254470.11683007282378796No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA253240.11626536582660457No Hit
CTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCT240730.11052188246500758No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC233820.10734942283042441No Hit
GTGGGGATGACGTCAAGTCATCATGGCCCTTACGAGTAGGGCTACACACG230070.10562775515608477No Hit
CCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGAGATCGTCGGCTTGG221320.10161053058262565No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGC218740.10042602322267999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTA324750.052.12221145
CTTACAT74350.047.3948361
CCATTGT522550.045.31892145
CCGGGAA765450.042.3036271
TGTACTC64200.040.9749832
TTGTACT65000.039.9342351
TTGAACG118450.039.8827
TCAGATT121450.039.083282
AACGTAT986300.035.758486
TTACATT96950.035.5800362
ATTGAAC139500.034.952516
GGAACGT990450.034.8155254
TGTGCCG132900.034.229443145
GTATTCA1034350.034.166119
ACGTATT1049900.033.9877747
GAACGTA1055300.033.5424735
GATTGAA149950.033.0949445
GGGAACG1064750.032.9326933
CGTATTC1099500.032.140158
CAGATTG162200.031.7576433
Produced by FastQC (version 0.11.5)