FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_6751.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_6751.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23276018
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT277530.11923431232954021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC121050.027.1105384
AGTCTAA132850.024.8681493
GAGTCTA150700.022.5040422
TCTAACA163750.020.3516185
GAACGTA131350.018.642715
GTTACGA44500.018.5587844
AACGTAT133300.018.3711786
TACGACT51700.016.9568546
TGCGCGA165850.016.3403959
ACGTATT149500.016.2358347
GTAGCAT105000.015.5261657
AGAAGCG133050.015.51796154
TTACGAC58300.014.9119755
CGTATTC175750.014.8010178
GTGCGCG183050.014.6461828
GAAGCGA145950.014.5440695
ATGACGT111900.014.4392867
TAGCATA112100.014.4142388
CGGGAAC174300.014.3848922
CCGAACG162600.013.2883511
Produced by FastQC (version 0.11.5)