FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_6215.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_6215.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19671731
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATC365190.18564202611351283No Hit
CGGGAACGTATTCACCGCGCCATGGCTGATGCGCGATTACTAGCGAATCC357400.1816820288972028No Hit
CGCGAAACCAAGTGATCTACCCTTGGTCAGGTTGAAGGTTAGGTAAAACT351480.17867263435027655No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC224950.11435190934646271No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT208680.1060811577791502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT113950.045.7515531
TGTACTC130750.039.9062772
GTACTCT146650.036.4094733
TCTAGTT151350.034.612277
TAGTTGT151900.033.7267539
CGACCTA39850.030.5410084
TGATCCT123200.030.5182114
AACGTAT475600.029.7362166
TACGACT97550.029.5669292
TACTCTA180250.029.4598944
CCGGGAA462300.028.6083131
CGTATTC500800.028.4889628
GAACGTA499200.028.4884745
CACTATA80900.028.2146649
GTATTCA522750.027.349289
GATCCTG140700.026.8266875
ACTCACT89500.026.7949336
AGAGCAT137550.025.6005867
ACGTATT571700.025.37297
CCATTGT422800.025.329123145
Produced by FastQC (version 0.11.5)