FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_6119.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_6119.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23529509
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA376980.16021583790804986No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC366410.15572360647219627No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG295350.12552323127524675No Hit
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC291280.12379348842340909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATTGT440100.034.948696145
CATTGTA282100.034.82267145
TACTACT55650.030.4655916
AACGTAT698150.029.8780236
CTTACAT63500.029.8028771
CAATTAG90800.029.4482469
CCGGGAA601100.029.2038061
AGCAATT80550.029.1446427
GTATTCA743650.028.1610079
GCTCAGA101950.027.3601575
GGAACGT671250.027.3386214
CGGGAAC654000.027.0695062
CTATTAG61900.027.041238
ACGTATT789800.026.724077
TTGAACG107950.026.378447
CGTATTC795650.026.2468388
GGTTAGA449800.026.1411534
GGGAACG794200.025.0693453
GATCCTG157250.024.9259475
GAGCAAT106300.024.33296
Produced by FastQC (version 0.11.5)