FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_5867.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_5867.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21430688
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC286090.13349548087303592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG94300.033.498745
ACGTCCG83050.027.693178145
CTATTAG90250.025.211348
TCAGATT128000.024.1185742
CCGGGAA446600.024.10961
CAATTAG73700.023.6962859
AACGTAT495000.023.5959156
GGCTCAG137200.022.9706154
CCATTGT363750.022.857819145
CATTGTA229500.022.540314145
CTGGCTC161550.022.4730229
TGGATGC167700.022.3797633
TTTGATC187850.021.9123612
CGGATGT48050.020.975081
GAACGTA565200.020.7666555
ACGTATT579650.020.3009557
CGTATTC600400.020.1311638
TCGATCT55550.020.0860923
TATTAGT120100.019.8510579
GGATGCC223600.019.7325384
Produced by FastQC (version 0.11.5)