FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_4340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_4340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19673317
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA28850.028.3762994
CCGGGAA341500.025.39351
AACGTAT406950.025.0314585
CAATTAG91550.024.5374989
CATTGTA259400.024.109673145
CGTATTC429600.023.6640077
TATTCAC456650.022.6764819
TTGAACG91900.022.549947
GATTGAA161350.022.4064715
GTATTCA458250.022.3280268
GGAACGT400900.022.0290763
TCAGATT106050.022.002952
CGGGAAC405350.021.8407541
GGGAACG416450.021.537063
GAACGTA493550.021.3136844
CCATTGT355850.021.224516145
GATCCTG113550.020.800435
TGATCCT116850.020.770134
ACGTATT508450.020.4631446
TCACGAG79800.020.1597822
Produced by FastQC (version 0.11.5)