FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_4340.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_4340.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24274672
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA103950.028.443172
CTTACAT119450.027.3170431
TTACATT118650.027.2401472
AACGTAT488600.025.2687726
GTTACGA70900.025.2393154
TCTAACA121650.025.0739565
GAACGTA517200.024.276885
CCATTGT342450.023.940943145
CGGGAAC496700.023.8396682
ACGTATT533300.023.4510027
CTCGGCT99400.023.4167181
AGTCTAA125750.023.3910833
CATTGTA244500.023.383385145
TAGCATA107250.023.3778259
CGTGTAT92450.022.601933145
ACATTCA144850.022.507174
GGTTAGA215000.022.2398764
GCTCAGA111500.022.2230725
GTAGCAT112950.022.197528
TGCCAGT115800.022.0906492
Produced by FastQC (version 0.11.5)