FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_3256.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_3256.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736709
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC313100.15863840319072445No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT292800.14835300049263533No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG219860.11139648459122542No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT203170.10294016089511175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT143950.051.2411547
GTACTCT153550.048.551323
TAGTTGT155500.047.3469859
TACTCTA161250.046.5452884
TTGTACT166600.044.999991
TGTACTC174150.043.0258452
TAATACG73050.038.2809524
ATAATAC73100.037.7609633
GAGCATT173000.034.1788
CACTATA109600.033.4556129
CTCTAGT231250.032.61566
AGCATTG181250.032.4243979
ATACGAC114800.031.3892861
ACGACTC120050.030.3557723
GTTACTA75850.029.8967673
TACGACT125850.029.3087222
ACTCACT126950.028.9926156
GACTCAC125450.028.528985
AGTCTAA41500.028.2814983
GAGTCTA41500.028.1131922
Produced by FastQC (version 0.11.5)