FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_3256.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_3256.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23905421
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA360130.1506478384128855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT174400.041.640976145
AGTGTAT141500.034.55706145
GATCCTG108250.028.8462185
GCTCAGA125700.026.8013825
CCGTATA53100.023.636593145
CGTGTAT106850.021.523752145
TGTACTC79650.021.4724732
GAAACTA178800.021.3937474
TTGTACT77250.021.2137151
TGATCCT151750.021.0060524
CGTGCGT76200.020.6350778
CATTGTA218200.020.547878145
CTCGGCT106600.020.474631
CTATTAG105500.020.12418
CAATTAG83500.019.7865319
AACGTAT393300.019.195436
TCCTGGC160750.018.9761417
CCATTGT297450.018.97575145
CTAACCA189000.018.7860558
TCGTGTT139900.018.66887145
Produced by FastQC (version 0.11.5)