FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_30982.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_30982.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20541903
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA1295020.6304284466731247No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA749380.364805539194689No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG496200.24155503022285715No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC461690.22475522350582613No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC416400.20270760698266366No Hit
GTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCACAGA386940.18836618983158473No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCG370330.18028027880377No Hit
CGGGTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGG354820.17272985857249934No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG345440.16816358250742397No Hit
GTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTC310000.1509110426624057No Hit
AAGAAATCAACCGAGATTCCGCAAGTAGTGGCGAGCGAACGCGGAGGAGC274110.13343943840061945No Hit
CCCGCCCTACTTTTCGTACGCTTAGTACCACTGTTGAGATTTCGAATACG259890.12651700283075037No Hit
GTCCCGCCCTACTTTTCGTACGCTTAGTACCACTGTTGAGATTTCGAATA257850.12552391080806877No Hit
TGAAGATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTA228980.11146971144786343No Hit
CGGAAAGACCCCGTGAACCTTTACTGTAGCTTTGCATTGGACTTTGAAGT222940.10852938016502171No Hit
GGGGGCTGCTCCTAGTACGAGAGGACCGGAGTGGACGAACCTCTGGTGTA222590.10835899672975771No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC214650.10449372679833994No Hit
ATGCAATCTACCCGCTGCTAGACGGAAAGACCCCGTGAACCTTTACTGTA214160.10425518998897033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCA196700.061.7652934
TCACGAG196150.059.5538642
AGCAATT246100.053.0847437
GAGCAAT276700.050.0403336
CAATTAG286650.048.233769
GCAATTA294350.046.9460148
TTACATT164450.046.621372
CGAGCAA288550.044.9938165
CTTACAT175350.043.9581
GCTCAGA126450.042.684815
CCGGGAA473300.042.6676831
ACATTCA192000.040.335954
TTCAGTA192650.040.2439277
CATTCAG194850.040.156815
GGTTAGA586100.039.949884
GGCTCAG143200.039.6127134
TGTCCTA141300.039.3268435
CTGGCTC138300.039.0885772
TTTCCGT382750.038.554947145
TACATTC202450.037.7538383