FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_30891.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_30891.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24051678
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT523420.2176230697916378No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC398040.16549365079642261No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT381290.15852947973110232No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT354250.14728702088893758No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT317830.13214462625019344No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC303740.12628640712718672No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT301700.12543823345714175No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG283940.11805413327086783No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA276650.11502315971467769No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC274130.11397541576932803No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC273360.11365527178602675No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG269490.11204623644138259No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA267620.11126874391050802No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG263140.10940608800766416No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA260810.10843734062962261No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG254860.10596350075865808No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT251960.10475776367869219No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC251600.10460808597221366No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC250030.10395532486340453No Hit
CGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTT246790.10260822550509782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA121750.053.9829442
AGTCTAA131000.050.3827933
GTCTAAC165050.039.7670564
TCTAACA167650.039.325555
GAAACTA259350.032.6541984
TAGCATA81250.032.0165758
GTAGCAT81600.031.4336157
TCGTGTT78000.031.34647145
GCTCAGA129350.031.2539355
GCCAGTA83600.030.765023
CTAACCA267250.030.611188
CGCTAGG27350.030.46154
AAGAAAC281550.030.0868852
CTCGGCT106950.029.8975981
GGAGTCT223000.029.815561
GTTACGA54350.028.3916634
TGCCAGT92500.028.1236042
AGAAACT303850.028.0403393
TTACGAC55500.027.9355835
GGCTCGT107100.027.8688144
Produced by FastQC (version 0.11.5)