FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_30887.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_30887.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20347466
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG408260.2006441490060728No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC400600.19687955247105465Illumina Single End PCR Primer 1 (96% over 33bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC393340.19331154061149433No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC359840.1768475740418979No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT352290.17313703829263064No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG307130.15094262843343736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA96800.057.18939145
ATAATAC104850.051.4115563
AGCATTA164050.041.6880384
GCATTAG165650.040.8066025
CATTAGA169550.039.913156
ATACGAC164850.037.6527521
TGTGTAT125600.035.988575145
GACATAC65350.035.4841429
TAATACG170550.032.4107064
CACTATA200550.030.5687479
TAGAGGT224500.030.1804149
AGGGAAG242900.029.9541072
AAGAGCA246200.029.8671444
GAGCATT253700.029.3017676
CAGCATT247100.028.8228573
TTGTACT133350.028.8200511
CTATGTT124300.028.309807145
TACGACT234900.028.1668262
GTAGATA393000.027.028055145
TCTAGTT148300.026.9212677
Produced by FastQC (version 0.11.5)