FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_30848.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_30848.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24600110
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG1093200.4443882568004777No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC998010.4056933078754526No Hit
CACACGTGCTACAATGGTTGGTACAACGAGTTGCGAGTCGGTGACGGCAA321780.1308042931515347No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC294340.1196498714843145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTA404700.042.705257145
CCGGGAA687850.038.5892831
CTATTAG109000.037.1597758
TTAGAGT215050.030.3214046
AACGTAT940900.029.7305246
GGGAACG960550.029.68643
GAACGTA952700.029.4809654
CCATTGT655800.029.285679145
GGAACGT961350.028.8313854
CGGGAAC965500.028.2355521
ACGTATT1014450.027.9545387
CGATCTA62200.027.9526464
TATTCAC1004700.027.6803919
CGTATTC1063200.026.572118
GTATTCA1076700.026.334129
GTTAGAG252900.025.724785
GGTTAGA286900.024.6950784
GGGTTAG295300.023.1105273
TATTAGT189950.022.1636099
CGAGCTA82150.020.3707264
Produced by FastQC (version 0.11.5)