FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_30785.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_30785.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22697381
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT438350.19312800890992665No Hit
CCCCACCATGCCGTAATGTACCGCGTTTTGATCCTGCTTAGGGCAGGTGG393260.17326228078913597No Hit
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC378990.16697521180967972No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT358440.1579213037839035No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA321190.141509718676353No Hit
GTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCGAACTCT315670.1390777200241737No Hit
GCGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGT293980.12952155140718657No Hit
CGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGTC270340.11910625283154914No Hit
GCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGT243260.10717536089295943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACAC144100.045.56761145
CTTACAT86250.041.021851
ATGCCGT315850.039.896068
GGCCATA90750.037.208652
CCATAGC89850.035.8786774
TTACATT100750.034.8819922
TACATTC104900.033.9094433
TTGGTCC184850.033.5900346
CCATGCC379350.033.0411456
CCACCAT297400.032.8857233
ACCATGC325300.032.8497545
GTAGATG147150.032.493523145
ATTCAGT125800.031.9640226
CCAAGTG228800.031.1004588
TGTGTAT58800.030.725126145
GTCCCAC206500.030.6701629
ACTCGAA84550.030.5899525
TAGACCT112450.030.2842737
TGCGTGT130950.030.2128522
CTCTATT103150.029.824163145