FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_30782.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_30782.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16065015
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG576260.3587049249564971No Hit
CCCCACCATGCCGTAATGTACCGCGTTTTGATCCTGCTTAGGGCAGGTGG375690.23385599079739422No Hit
CCCACGAGTGCCGTTTAGGCTGAATCCGCTTGAACCTTGCTGACAAGGCG327940.20413301823870067No Hit
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC249560.15534377029837818No Hit
GTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCGGACTCT229310.14273874005097412No Hit
GCCTTTCACCCCTATCCACAGCTCATCCCCGCATTTTGCAACATGCGTGG219420.13658250552520493No Hit
CGCGAAGGCATATATTCCTTTGAACCAATACGTTCGCTTTAGGTTACCGG201110.12518506829903364No Hit
GCGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGT175460.10921869665232183No Hit
GTGCGTGTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCG171860.10697780238611666No Hit
CGACTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAA170460.10610634350481465No Hit
CCGGCGATGACCTACTCTCCCACACCCACAAAAGTGCAGTACCATCGGCG169740.10565816465157361No Hit
GTTCGCGAAGGCATATATTCCTTTGAACCAATACGTTCGCTTTAGGTTAC164490.10239019384669107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT147350.058.160621
TACATTC156150.055.3515933
TTACATT156050.054.934422
ACATTCA167850.051.8774074
TTCAGTA168850.050.547887
AGATTAT95500.049.461765145
CATTCAG185200.046.5107775
ATTCAGT186900.046.090456
TCAGTAG186100.045.8256958
ACGAGTG125550.043.1021584
CAGTAGC200500.042.5361949
CACGAGT133250.040.3430143
TGTCCTA134650.040.1370245
TACGACT99250.039.645412
ATATATG98700.037.051464145
CGAGTGC158500.036.8399055
GAGTGCC165100.036.422476
ATCACAC140700.035.68651145
AGTGCCG170050.035.0656477
TCCGAGT74600.034.5807762
Produced by FastQC (version 0.11.5)