FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_30782.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_30782.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814874
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA152450.026.69938145
GTACTCT104000.022.0822723
CGTGTAT103700.021.479559145
CTCTAGT110450.020.9254346
TACTCTA111900.020.3932344
ACTCTAG123450.020.070595
CTAGTTG117900.019.359548
TGTACTC131450.017.1452752
TCGTGTT130750.016.75834145
TAGTTGT138500.016.2714399
TTGTACT141450.015.4799461
TCTAGTT153600.015.18927
CCGTGTT137200.013.53791145
GTAGATA174150.012.581987145
TACGACT83050.012.0415972
CGACTCA94000.011.0208194
GACTCAC99750.010.5312585
CACTATA86850.010.5127449
GAGCATT151100.010.4062348
TAATACG66950.010.2796584
Produced by FastQC (version 0.11.5)