FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n02_MT_2397.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n02_MT_2397.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21540450
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA71400.048.905694
TCGATCT75550.044.3032073
ATCTATT101200.037.1573526
CTATTAG134500.033.240558
TGCCGTA224400.030.7748249
TTCAGTA87950.028.9168787
ATGCCGT236000.028.7081328
GTGATAT176050.027.1812531
AACGTAT474700.027.1528036
CCATGCC250800.026.7799726
TCTATTA143950.026.677457
CTCGATC130950.026.6163182
ATTCAGT110350.026.5913666
GTATTCA488800.026.5515549
CCGGGAA437600.026.5096681
TGTCCTA84950.026.3529265
CTTACAT95600.026.0892831
ATTAGAA74050.025.8307366
TTACATT98350.025.2713322
TATTCAC516250.024.901139
Produced by FastQC (version 0.11.5)