FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27182195
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2091570.7694632460697158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA367330.13513625371313834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAC238500.022.4210172
CCATTGT169500.022.224554145
CCGGGAA238700.022.0663171
GTATTCA269750.021.3708789
GAACGTA272700.021.3520345
GGAACGT265550.020.481514
GGTTAGA160950.019.9783844
TACGACT71350.019.2950972
TATTCAC257250.019.2843919
CTTACAT75450.018.031261
GGGAACG336800.017.639163
CAGTCTA70100.016.96768425-29
CAATTAG59000.016.6938939
TGTACTC48450.015.4039032
GATCCTG95700.015.3621665
AACGTAT398300.015.2370926
TTGTACT47900.015.0363821
TTAGAAC174000.014.8589246
TAGAACC144000.014.7860597
GCTCAGA71400.014.5046155
Produced by FastQC (version 0.11.5)