FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_9361.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_9361.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20525173
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG464940.22652184222759048No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG304440.14832518098629424No Hit
CCCCACCATGCCGTAATGTACCGCGTTTTGATCCTGCTTAGGGCAGGTGG294420.1434433707330993No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC291540.14204021569026481No Hit
GTGATATCGCAGTTTTGAGCCAACACATTTACTTCGGGAGTCCGAACTCT233310.1136701746679553No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA220290.10732674457847444No Hit
GGAAGAGAAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAG209720.10217697068862708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACATT158500.045.4638442
ACATTCA163700.044.1964844
CTTACAT182500.039.7286031
TTCAGTA199850.036.492177
CTCATAT36650.033.6311041
TACGACT106700.032.9523472
CATTCAG226250.032.9069565
TACATTC222300.032.643863
CAGTAGC227100.032.1771979
CTATTAG84300.030.70058
TCAGTAG248950.029.1783288
CATTGTA257700.028.975277145
CCGGGAA317300.028.5861
CACGAGT106350.027.5391653
ATTCAGT281650.026.8460486
ATATCGC137400.026.4863954
TGGTTCG156600.025.6463133
CAATTAG69150.025.5801129
TTGTACT41400.025.569291
TGTACTC40050.025.5226422
Produced by FastQC (version 0.11.5)