FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_9109.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_9109.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21767998
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAACGCATAGAAGTGAGAATGCCGGTATGAGTAGCGAAAGACAGGTG540810.24844269096312854No Hit
CTCGGTTTCCCTACGGCTCCGTCTCTTCAACTTAACCTCGCATCATAACG500770.2300487164690111No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC497210.22841328816733628No Hit
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG419990.19293919450010974No Hit
GGAAGAGAAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAG400030.18376977065139383No Hit
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC346080.15898568164146284No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG292650.13444047541716972No Hit
CTGGGTTAGAGTGGCCATAACACAAGGGTAGTATCCCAACAACGTCTCCT257670.11837101418329789No Hit
GGAAGACGCAGTGAACTGAAACATCTAAGTAGCTGCAGGAAGAGAAAGCA255230.11725010265068933No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG254770.11703878326339426No Hit
GTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACG227800.10464903570829068No Hit
GCCGGTATGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACT226000.10382213375800568No Hit
GAGAAAGCAAAAGCGATTGCCTTAGTAGCGGCGAGCGAAACGGCAGAAGG217870.10008729328255174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT95200.047.903331
CTATTAG76950.041.6405838
CCGGGAA483450.041.4363371
TTCAGTA111450.040.979317
TACGACT86950.038.686062
CCATTGT388800.037.23531145
AACGTAT633050.035.522946
ACATTCA132000.035.2037734
CGGGAAC593900.034.3248062
TACTACT45050.033.6323056
GTATTCA681200.033.3205039
CAATTAG63550.032.511169
GAACGTA694750.032.4934045
ACGTATT704350.032.359237
GGAACGT658400.032.2944154
GGGAACG664950.032.0090073
CATTCAG153800.030.8266685
CATTGTA311550.030.668377145
CCCTACG375000.030.2155749
CTCGGTT357650.030.144271
Produced by FastQC (version 0.11.5)