FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_9109.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_9109.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8226689
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT103850.12623547577889477No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC93490.11364231709743737No Hit
CGAACTCACACATAAGTGTTGACCGACTATTTTTGTATTATTAGGGAAAC82330.10007671373015316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC22900.033.556522
CACTATA60850.030.3798859
TACGACT64000.030.3571782
GACTCAC65700.030.1232055
CACATAA47100.029.7059179
ATACGAC63500.029.5737551
CGACTCA67700.029.1262234
GTTACTA37100.027.5519143
GTACTCT62450.025.7707523
GTACGAA31800.025.081071
TTGTACT62300.025.022511
ACTCACT78400.024.7813686
ACACATA59750.024.6300148
CTCACTA76850.024.5265257
TTATGTG32450.024.127535145
GAATAAT59450.024.0267371
GAGCATT58100.023.9569578
TGTACTC66050.023.9271092
ACTCACA58600.023.628694
AACGTAT83600.023.3266626
Produced by FastQC (version 0.11.5)