FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_8337.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_8337.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19739353
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA35800.041.309524
TCCTGGC146700.034.4927027
ATCTATT48550.032.402136
GATCCTG198850.030.331985
CCTGGCT169400.030.298478
AAAATCG32200.030.1684553
TGATCCT226850.027.2272534
GTGATAT91400.025.066741
GGGAAAT53900.024.4815881
TGTCCTA63650.023.4623665
CCAAGTG128050.023.4380558
CTGGCTC215650.023.2961759
CATTGTA225400.023.156965145
ATTAGAA62950.023.1474636
CAAGTAT44000.023.0662088
AAATCGA51050.022.436984
AATACTA47350.022.1999024
CGTGCGT47400.022.0234328
CGCTCAG39550.021.99574
CTTTTAC61650.021.8744341
Produced by FastQC (version 0.11.5)