FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_7431.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_7431.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24922654
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCG608160.24401895560561085No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC570550.22892826743090844No Hit
CGGAAAGACCCCATGAACCTTTACTGTAGCTTTGCATTGGATTGCGAACC316420.1269607963903042No Hit
CTAGGTTAGAACCGCGAACAAACCAGGGTGGTATTTCAAGGATGGCTCCA273350.10967933029925303No Hit
CCCGAAACCAGGTGATCTATCCATGGTCAGGATGAAGGTTGGGTAACACC272810.1094626599558779No Hit
CACCAACTAGCTAATCTGCCATCGGCCGCTCCAATAGCGCGAGGTCCGAA261410.10488850826240255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA22650.058.2511524
TGATCCT192450.044.4870154
GATCCTG213700.041.250615
GCTCAGA115050.037.8694655
TCCTGGC230100.037.2392857
CTGGCTC239100.035.5342149
CCGGGAA506900.034.270111
CATTGTA364900.034.051456145
TGTCCTA89050.033.540125
CCTGGCT259100.032.8752568
GGCTCAG225150.030.0407684
AACGTAT709000.029.4065445
ATCCTGG299400.029.2251536
CAATTAG109150.028.359789
CAGATTG145800.028.0924538
TTTGATC318500.027.4272612
GTATTCA759900.027.3507948
CCATTGT607750.026.981514145
TTCAGTA96000.026.7320617
CGTATTC792200.026.33647
Produced by FastQC (version 0.11.5)