FastQCFastQC Report
Wed 26 Jun 2019
HHG5CBGXB_n01_MT_6751.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHG5CBGXB_n01_MT_6751.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21781207
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCC1361320.6249975035818722No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG1041840.47832059995573245No Hit
CGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATT699610.32119891243859905No Hit
CGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGA514460.23619444046420385No Hit
TGAAGATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTA468360.21502940585432204No Hit
CTTTACTATAGCTTGACACTGAACATTGAATTTTGATGTGTAGGATAGGT397140.18233149338326382No Hit
GCCCGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATT375880.1725707854482077No Hit
ATGCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATA320540.14716356168875305No Hit
GCGGTGTACCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGC310590.14259540345950525No Hit
CCCCAGTCAAACTACCCACCAGACACTGTCCGAGACCACGTTTCGTAATC306870.14088750912656034No Hit
CACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGCTGGA288000.13222407738928335No Hit
CGTGAACCTTTACTATAGCTTGACACTGAACATTGAATTTTGATGTGTAG287060.13179251269224887No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAA275600.12653109627946696No Hit
GCTACACACGTGCTACAATGGCGTATACAGAGGGAAGCGAGAGTGCGAGC264330.12135691102885163No Hit
TGGGAATCTAGCTTATGGAGGGGGATAACTACGGGAAACTGTAGCTAATA257950.11842778042557513No Hit
CCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGAGATCGTCGGCTTGG251330.1153884630911409No Hit
CCCGCGGCTAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTGACACT247960.11384125774113436No Hit
GGAAAGACCCCGTGAACCTTTACTATAGCTTGACACTGAACATTGAATTT242130.11116463839676102No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG231760.10640365338798717No Hit
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC223940.10281340239776428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTA331100.050.008495145
CTTACAT73050.049.327931
CCATTGT521650.042.45598145
CCGGGAA735850.039.993231
TTGAACG116600.039.7913137
TCAGATT117600.039.576522
TTACATT96200.037.07662
CATTATG110000.033.94075145
AACGTAT956500.033.7273256
ATTGAAC140850.033.712536
CAGATTG153600.033.0383
GGAACGT958250.032.984854
GTATTCA998000.032.4265339
GATTGAA149750.031.9025555
ACGTATT1026700.031.7319227
GAACGTA1023200.031.705855
CCATATT89750.031.2598132
GGGAACG1051200.030.5027143
CCCATAT91800.030.3281061
CGTATTC1075300.030.1157178
Produced by FastQC (version 0.11.5)